Update on Anti-Aging Developments

by Bob Seitz

April 22, 2006

First, the Good News:

The Pavement-Pounding Pace of Anti-Aging Progress

A lot has happened in the last two months. Perhaps the “goodest” of the good news is that anti-aging developments, including cheap, simple ways you can avail yourself of them, seem to be happening fast. It reminds me of the period in 1944 just after the unveiling of the V-2, or the crescendoing announcements in personal computers from 1974 onward Here are some specific developments over the past two months.

(1) Resveratrol Fed to Killfish Extends Their Maximum Life Span 59%

Italian researchers, in a paper in Current Biology[1], have shown that killfish fed resveratrol had a maximum life span 59% longer[2] than a normally fed control group. Killfish are small fish with three-month life spans.

This is important because it’s the first experiment to show that orally administered resveratrol will increase maximum life span in a vertebrate.

Following Dr. David Sinclair’s landmark 2003 discovery that resveratrol appears to be the number-one long-sought calorie restriction mimetic, it was quickly shown that resveratrol is almost immediately metabolized in humans, and it was argued that resveratrol would be ineffective as a calorie restriction mimetic. But apparently, ingestion is an effective way to administer resveratrol to animals.

Can resveratrol’s calorie restriction effects be added to calorie restriction itself, deepening the level of calorie restriction beyond what would be practical by cutting calories alone? (Calorie effects are proportional to the degree of calorie restriction that is being practiced.)

In any case, resveratrol could be a worthy addition to life extension strategies. 

One note of caution:  The authors of the resveratrol paper point out that there was an initial increase in morbidity in their fish. They suggest that resveratrol might be somewhat toxic. Presumably, red wine extract should be safe in moderate doses. Still, caveat emptor….

One question is:  how do you translate doses of resveratrol killfish to equivalent human doses? One day in the life of a killfish would equate to about a year for us. Do we divide the maximum-longevity killfish dose of 150 mg./kg. of body weight by 365? That would give us about 0.4 mg./kg for the human-equivalent daily resveratrol dose, or about 24 mg. (approximately what’s in Longevinex’ resveratrol) per day for someone who weighs 60 kg. (132 pounds).

If resveratrol and calorie restriction increase life spans additively, then the long awaited, over-the-waterfall chain reaction in the conquest of aging might possibly be at hand. Surely sometime soon, the public is going to awaken to the existence of something (CR and its mimetics) that can virtually eliminate the chances of artery diseases, substantially delay the onset of degenerative diseases, including arthritis, preserve youthful appearances, and extend life spans by a decade or two. And that, I think, is going to trigger a runaway effort to conquer aging.

(2) Calorie Restriction Improves Diastolic Function in Humans

The Washington University researchers who reported in a National Academy of Sciences paper[3] in April, 2004, that a complement of 18 calorie restricted subjects were exhibiting dramatically improved cardiovascular risk profiles has followed up that original paper with a follow-on study[4] in the Jan. 17, 2006 Journal of the American College of Cardiology. This paper shows that the diastolic functions of the hearts in 25 calorie-restricted individuals was, on average, about 17 years younger than that of fully fed matched controls.

The cardiovascular risk factors examined in the first (2004) Washington University study are secondary biomarkers of aging. They are parameters that tend to increase with age, but they are amenable to medications and lifestyle changes that will, for example, lower the levels of total cholesterol and raise the levels of HDL. Diastolic function, on the other hand, is a primary biomarker of aging, and is a measure of the elasticity of the heart.  It’s (to the best of my knowledge) unaffected by exercise, and steadily declines over the course of a lifetime. Improving diastolic function might be construed to be a partial reversal of aging, at least insofar as diastolic function is concerned. One interesting point that was elucidated at the conference: CR decreases the stiffness of blood vessels by shrinking cartilage that develops around blood vessels with age rather than by breaking up cross-linkages. Promising natural cross-link breakers are carnosine, benfotiamine, and pyridoxamine (one of the three vitamers that comprise vitamin B6).

A recently completed Washington University study, Calorie Restriction Appears Better Than Exercise At Slowing Primary Aging, reveals results that are, perhaps, more fundamental and important then the cardiovascular measurements of calorie restricted humans that Washington University researchers have unearthed. This latest study showed that the calorie-restricted have lower levels of tumor necrosis factor (a biomarker of inflammation), and T3 (triiodothrronine) (though not of thyroxin-T4- and thyroid stimulating hormone-TSH). Lower levels of T3 mean lower body temperatures, lower cellular body weights, and to some extent, free radicals. The CRANies also have much lower levels of insulin (by a factor of 3 or 4) and much lower levels of Insulin Growth Factor 1, all of which are found in calorie restricted animals, and all of which suggest a reduction in the rate of primary aging. This latest Washington University study also examined endurance athletes with the same body fat percentages as those on calorie restriction. The endurance athletes ingested 2,700 calories a day versus 1,800 calories a day for the calorie restricted group.  In spite of the fact that the endurance athletes had the same body fat levels as the CRANies, their average level of T3, while lower than the average for the control group, was closer to the average T3 level of the control group than to that of the calorie restricted group. The same situation occurred with respect to triglycerides and HDL. C-reactive protein, fasting glucose, and blood pressure levels are also much lower only in the calorie restricted.

Another anti-aging supplement that might make a difference is the slow release form of the R+ enantiomer of alpha-lipoic acid[5].

(3) Other Follow-On Studies of Calorie Restriction in Humans

Capitalizing upon the success of the original Washington University pilot study, the National Institute on Aging has funded four parallel succeedanea with Washington, Tufts, and Duke universities, and with the Pennington Biomedical Research Center in Baton Rouge, Louisiana, in a program called CALERIE. These two-year studies will be completed within the next couple of years.

(4) Protandim, GliSODin, and SODzyme

Last year, Tommie Jean called me to watch an ABC TV program extolling a new breakthrough in aging. It was about something called Protandim[6]. Protandim is the trade name for a capsule that boosts the endogenous production of Superoxide Dismutas (SOD), Catalase. and Glutathione Perioxidase. Superoxide dismutase, catalase, and glutathione perioxidase are the body’s own primary antioxidants. Previous attempts to deliver these endogenous antioxidants in supplement form failed because they’re unable to survive the GI tract. Unfortunately, their levels decline with age, rendering us ever more vulnerable to free radical damage. For decades, people have taken vitamin E and vitamin C for antioxidant protection, with negligible results.  Apparently, boosting vitamin C and vitamin E levels doesn’t affect health unless an organism is deficient in them.

In 1954, Denham Harman, at the University of California, Berkeley, propounded the free radical theory of aging.  Could aging be caused entirely by free radical damage accumulating over a lifetime? Researchers began to feed antioxidants to laboratory animals hoping to slow their rates of aging.  Alack and alas! Although feeding antioxidants to lab animals extended their average life spans (by 30% in the graph shown above), they didn’t seem to affect the underlying rates of aging or extend maximum life spans.

The take-home message was that something else—maybe genes—are controlling the aging process.

Recently, Joe McCord, the co-discoverer of superoxide dismutase, identified five botanicals that elevate the levels of these intracellular antioxidants to values appropriate to young adults, eliminating these antioxidants’ age-related declines[7].  The five botanicals are turmeric, 75 mg., green tea extract, 75 mg., silymarin (milk thistle seed), 225 mg.; bacopa, 150 mg.; and ashwagandha, 150 mg. Their SOD/catalase-stimulating effects are thought to be additive rather than synergistic. Dr. McCord chose using small additive doses of five SOD-enhancers rather than one large dose of any one of them because (I suspect) he was conducting the moral equivalent of Phase I and Phase II trials, and didn’t want to risk possible side effects brought on by a large dose of any one of the ingredients. The point is; you can probably sprinkle turmeric on your food and drink green tea and get the same effect. However, I should also mention that there’s a minimum and maximum effective dose for this, and 675 mg. of Protandim is probably somewhere close to an optimum dose. (You have to be careful. Too much of this can promote oxidation rather than reduce it.)

Turmeric is also a potent anti-inflammatory like aspirin.

In a small pilot study involving about 20 subjects, Dr. McCord found that Protandim reduced the levels of oxidative stress in his subjects to youthful levels. 

The Life Extension Foundation says, “SOD, for example, may be up to 3,500 times more potent than vitamin C in quenching the dangerous superoxide radical.[8]” The reason is that vitamins C and E quench free radicals stoichiometrically by sacrificing a molecule of vitamin for every free radical quenched. The cell's antioxidants operate catalytically, neutralizing free radicals without being consumed in the process.

Quantitative differences of this magnitude can become qualitative, and this is what Dr. McCord is hoping. There are some animal experiments involving transgenic animals modified to overexpress SOD or catalase, and in both cases, show significant maximum life span extensions.  However, the touchstone will probably be life span studies feeding Protandim to mice. We might want to go slow with this until that takes place.

There are societies in which turmeric is widely consumed, and societies in which green tea is widely consumed and some societies in which both are ingested each day, and yet, the members of these societies don't, to the best of my knowledge, live dramatically longer than members of other societies. There may even be individuals in India who consume turmeric, green tea, and one or more of the other three herbs: milk thistle seed, bacopa, and ashwaganha.

 Meanwhile, back at the North Pole,

Santa’s little elves were busy. While Dr. McCord was toiling away with Protandim, a French company had discovered a melon that would keep without refrigeration for several weeks, unlike your ordinary, proletarian melon that was only good for few days. It turns out this “super-melon” is loaded with superoxide dismutase. The French company found a way to get this SOD past the lining of the gut and into the bloodstream. They patented and trademarked their product, calling it GliSODin. At the same time, the Life Extension Foundation also developed a delivery system utilizing wheat, corn, and rice glutens, which they’ve dubbed SODzyme. GliSODin and SODzyme differ from Protandim in that they directly supply SOD to the blood stream, whereas Protandim stimulates the synthesis of SOD, etc., by the cell.

Safety Issues

Although turmeric and green tea have been around for thousands of years, that doesn’t necessarily make them safe. Who would have thought 60 or 70 years ago that coffee cream, butter, and eggs could be harmful to your health? I’m not aware of much in the way of safety studies of the long-term effects of turmeric (cumin) and green tea.  And if that’s true of such venerable tisanes as teas, think about resveratrol! David Sinclair is said to have muttered, in 2003, veiled cancer warnings about resveratrol. It was hard to know how to assess his alleged remarks because at that time, Dr. Sinclair was raising $45 million in venture capital to found Sirtris Corporation in order to try to develop and patent synthetic resveratrol analogs so that in a decade or so, they could be used as prescription drugs to combat diabetes, heart disease, and/or other debilitating diseases. However, Dr. Sinclair and his co-workers have launched life span studies of resveratrol in mice that should mature within a year or two.  Accordingly, after reading what I’m writing, I’ve decided to quit taking resveratrol (red wine extract) until a bit more safety information is available regarding its consumption. As red wine extract, it should be relatively safe, but we (or at least I) don’t know the proper dosage levels in humans.

U-Shaped Curves

The effectiveness of many pharmacological agents is described by a U-shaped curve. Too little is ineffective. Too much can sometimes worsen the condition that the pharmacological agent is trying to alleviate. There’s a happy medium.

Metformin

As I’ve mentioned before, in gene chip studies performed in 2001 by Dr. Steve Spindler at the University of California, Riverside, metformin, alone among diabetic drugs, generated most of the same gene chip changes as CR. A 1980’s life span study in mice used a metformin precursor called phenformin to extend the maximum life span of the mice by 23%. A quick, recent life span study of metformin in mice at the National Institute on Aging indicated a maximum life span extension of about 20%, and an ongoing life span study of the life span effects of metformin on mice sponsored by the Life Extension Foundation is slated to report its results by September, 2007.

Why Anti-Aging Pills Might Not Be Altogether Practical

If metformin is a CR mimetic, I began to wonder why it isn’t used off-label for such purposes as elevating HDL.  Then it hit me. Medicine is (necessarily) very conservative. Metformin’s predecessor, phenformin, caused 60 deaths in a million patients from lactic acidosis, and was withdrawn from the market. Its proponents said that a handful of prescribing physicians failed to warn their patients about feelings of extreme fatigue and muscle weakness, but phenformin was taken off the market just the same. Its successor, metformin, is safer (or maybe prescribing physicians are simply more aware of its possible lethal side effects), but patients and doctors are still advised to be on the lookout for adverse conditions. What this means is that safe dosage levels are going to be held down low enough that drugs allegedly kill, at most, a few patients per million. I say “allegedly” because (1) the death of someone who’s already very sick might be falsely attributed to metformin if it could justify a multimillion-dollar lawsuit, and (2) we’re all different, and different people will respond to metformin with different sensitivities. If we’re experimenting with mice, we don’t care if it kills 2% of our experimental arm, but if we’re experimenting with humans, we won’t accept a 0.001% lethality rate. And what’s true for metformin may be true for all CR mimetics and anti-aging pills.

For human safety reasons, the medical community may need to keep recommended dosage levels of anti-aging drugs too low to realize most of their potential benefits!

(5) My Bloodwork

I’ve previously mentioned the dramatic rise in my HDL in February, 2004, after I had been practicing calorie restriction (as in “losing weight”) for six months. Since then, my family doctor hasn’t seen it necessary to retest these parameters. Consequently, earlier this month, I purchased a blood chemistry/CBC test through the Life Extension Foundation.  I wanted to see if my dramatic rise in HDL had been maintained. (Statins will lower levels of LDL but they won’t usually raise levels of HDL Drugs that will elevate HDL are current targets of developmental opportunity among pharmaceutical companies.)

When my results came back, my HDL this time was 84, up 65% from my pre-CR days, and the ratio of total cholesterol to HDL was slightly less than 2.4, down 40% from my pre-CR days. (In the Framingham Heart Study, it’s alleged that no one with a TC/HDL ratio of 2.5 or below ever had a heart attack.)

Apparently, calorie restriction is still working.

Calorie restriction also lowers other inflammatory cardiac and cancer risk factors such as homocysteine and C-Reactive protein.

And Now, the (Possibly) Bad News):  

Aubrey de Gray's Presentation:

From April 5 through 9, I attended the Fourth Calorie Restriction Conference in Tucson, Arizona. One shocking message from the speakers was that CR may not slow aging in humans, and if it does extend maximum life spans, it’s by no more than two or three years.  Several of the speakers seemed to be inclined toward this outlook. Cambridge University's Aubrey de Gray, in a presentation entitled, "The Unfortunate Influence of the Weather Upon Human Lifespans", presented his hypothesis that CR should yield minimal life extensions in humans. Dr. de Gray began by observing that the early deaths of animals from predation precludes late reproduction. Animal species must reproduce early. But early reproduction requires rapid growth, and rapid growth goes with rapid aging and cancer. (Whoa! What about predators at the top of the food chain, like the big cats and grizzly bears? What about whales? Also, red sea urchins and rough-eyed rockfish reach sexual maturity early but live and continue to reproduce for at least 200 years. ?) Next, Dr. de Gray noted that starvation of worms multiplies their effective lifespans by a factor of three. Fruit flies go into diapause with the onset of winter to achieve a similar three-to-one ration in lifespans. Grasshoppers go into diapause during an arid summer. "So why are lifespans in mice increased only 40%?" Dr. de Gray observed that calorie restriction is designed to help animals survive a drought or a famine, and very few droughts or famines last 10, 15, or 20 years. A year or two is the longest most droughts or famines are apt to last. evolution will tend to provide no greater capabilities than the long-term environment requires ("use it or lose it"). If an organism only needs to survive for an extra two or three years, then over time, excess capacity will be bred out of the organism.   So  two or three years is probably the greatest life extension that humans can expect to experience with CR. 

Dr. de Gray then pointed out that CR has lengthened the average lifespan of Okinawans by less than two years over the average lifespan of the Japanese. (Michael Rae observed from the audience that the Okinawans were on 20% CR only until the 1960's and that they suffered from severe dietary deficiencies and had no modern medical care. Since the 1960's, they've become overweight junk food addicts.) Dogs on 25% CR experienced a maximum lifesapn extension of only about 10%. It's clear that calorie restriction improves the health of monkeys and humans, but major lifespan extensions...? It appears that CR increases lifespan by something like the same amount rather than by a percentage. 

Others Who Have Reached Similar Conclusions: Eric Le Bourg:

Eric Le Bourg at the Centre de Recherche sur la Cognition Animale observes that species that are able to migrate (including humans) would not be expected to respond to calorie restriction because it would be easier for them to migrate to better feeding grounds than to hunker down and adapt through caloric restriction. Dr. Le Bourg begins with the assertion that CR doesn't extend the lifespan of the common housefly or the Mediterranean fruitfly. He and a colleague have called into question the reported CR-induced, lifesapan extensions of drosophila melanogaster. He cites Ed Masoro and Steve Austad who postulated in 1996, that species not facing unpredictable short-term food shortages could have lost their ability to respond to DR*. For instance, vegetation-consuming monkeys living in tropical forests should not show a longevity increase when subjected to DR. since their environments are quite stable all year and year after year."  (I would add to this pelagic species such as tuna that live in the stable environment of the world's oceans, and that would have the ability to migrate long distances if local problems arose, although it's hard to imagine much variation in oceanic environments.) He then notes that "no positive effects on longevity in Rhesus monkeys has been observed by Ingram, et al. (2005): at least 12 years after the onset of DR experiments (17 years for some animals, n > 80), 50% of the control monkeys had died, the very same percentage being observed in monkeys subjected to DR*. Such a result is clearly not in favor of a positive effect of DR on human longevity, provided that monkeys and humans would respond similarly to DR."
              * - DR = "Dietary Restriction"

Others Who Have Reached Similar Conclusions: Lloyd Demetrius:

Lloyd Demetrius, a Harvard associate, has reached the same conclusion (that CR will have little effect in humans)  following a different line of reasoning. He argues that the response to CR is based upon an organism's metabolic stability. "Mice are not small people", Dr. Demetrius argues. Mice are an opportunistic species whereas humans are an equilibrium species. "In an opportunistic species, evolution will result in early sexual maturity, large litter size, and metabolic flexibility due to the high vulnerability to random changes in metabolic networks. In an equilibrium species, evolution will result in late sexual maturity, small litter size, and metabolic robustness due to the relative insensitivity of the system to random changes. The effect of CR on the two species will then be different. If we make the hypothesis that CR increases the stability or robustness of the metabolic network by increasing the metabolic efficiency and enhancement of homeostatic regulation in cells, we can predict that CR will induce large changes in an opportunistic species by increasing its robustness, whereas it will have weaker effects on an equilibrium species, in which robustness is already achieved. From this, Dr. Demetrius predicted that the response to CR with an increase in lifespan will be marginal in humans owing to the robustness or stability of their metabolic networks and the evolutionary history of the species."

Some Projected Consequences of These Theses:

Other Factors That Might Affect Life Spans Besides Droughts and Famines:

The Effects of Inhabiting the Tropics Versus the Temperate Zones

The turns of the seasons would seem to force most temperate animals to grow fast within a year. In the temperate zones, oestrus and birthing occurs in the spring so that new animals will be sufficiently mature to weather the harsh winters. Animals must store up fat during the summers to help them survive food shortages during the winters when nothing edible blooms and snow may cover the ground. This is in contrast to the tropics, where, food is available all year around.
Bottom Line: Temperate-zone animals must grow up in less than a year whereas tropical animals don't face an annual reckoning.

 


The Effects of Territoriality Upon Animal Migration:

Many animal species, including humans, might have been biologically capable of migrating away from environmental hardships, but this would have required moving into the territories of other animals, triggering conflict. If times grew harsh in Quebec, Algonquins who migrated into New York State might have triggered war with the Mohawks, the "keepers of the Eastern Door" for the Iroquois. If times were bad enough for the Algonquins to try to move south from Quebec, they probably weren't flush for the Mohawks.

Similar behaviors might have been evinced by other predators attempting to move into another predator's territory.

The Malthusian Predation Cycle:



Birds:

Birds in general, and large passerine birds  such as wild geese, in particular, reproduce each spring, giving birth to 3 to 6 chicks each spring that must reach maturity by fall in order to migrate south with the flock. Canada geese live up to 24 years in the wild. Does that make geese an opportunistic species? Their wild populations can increase by 10% to 17% a year, a fact that's currently threatening an unwanted population explosion. (This is in contrast to human populations that might typically increase as much as 3% a year.) (It's worth noting that anthropologists It would seem to me that Dr. Demetrius' argument would put wild Canada geese closer to mice than to humans.  On the other hand, since they can migrate long distances to escape deleterious conditions, Dr. Le Bourg probably wouldn't expect them to evolve CR.

 

 

 

One of the experiments cited in support of this thesis was the “Vallejo” study conducted in Spain from 1955 to 1958.

The Vallejo Study

 Sixty healthy individuals aged 65 and up (with an average age of 72) living in a religious home for the elderly, were put on alternate-day calorie restriction, ingesting 2,300 calories on one day, and 900-calories (of fruit and milk) on the next. This CR contingent was compared with a matched control group of 60 normally fed residents who received the full 2,300 calories every day. At the end of three years, 6 (10%) of the calorie-restricted subjects had died, compared with 13 (22%) of the control group, but this wasn’t statistically significant because the numbers were so small (6 and 13). In other words, the mortality rate in the CR group was about half that of the control group. The calorie-restricted group had registered a little over half the trips to the hospital experienced by the control group. This was statistically significant, because the numbers of occurrences were much larger.

I inferred that the carry-away message from this experiment was supposed to be that the subjects on the calorie-restricted diet didn’t live 10 or 15 years longer than the controls. But the study only lasted for three years. What would have happened if they had continued the study until the patients in both groups had all died? CR obviously helped the people who were on CR, but they would also have had pre-existing conditions such as atherosclerosis or occult cancer. CR might have raised their HDL levels and lowered their cholesterol levels, but it would have required several years for the full effects of CR to  kick in. Also, we know today that the transition to a CR diet must take place slowly in the elderly. Then, too, their nutrition wasn't what we might prescribe for them today..

CR roughly halved the mortality rate and cut the hospital visits in half.

What would have happened if you had started with 50-year-old health enthusiasts?

The Most Senior CRANie of Them All

Ralph Cornell, of Massillon, Ohio, began calorie restriction in 1953 when he was 50 years old. He’s now 103. Until two years ago, when he was 101, he drove to and from his real estate office every day. (I believe he ran a mile in 2004.) Then he fell and broke his hip (osteoporosis?), and now needs a walker to get around. He’s also had a heart attack.

He’s part of Paul McGlothin and his wife’s extended family, so Paul knows Ralph quite well. Paul says there may be some extenuating circumstances surrounding Ralph’s failing health. After all, he was the first, and issues of nutrition and medical care would have broken new ground.

Ralph’s relatives lived into their nineties, so he has good genes. The odds of reaching 103 from 95 through the luck of the draw are about one in ten. 

I have to admit that clean living and exercise could have given Ralph his centenarian status without invoking CR.

The Purina Study of Labrador Retrievers

In 1987, the Purina company initiated a small study of 48 Labrador Retrievers, 24 of which were put on 25% calorie-restricted diets starting at 8 weeks. The study lasted 15 years until the last dog was hanged—uh, buried. The control group was slightly restricted from the age of 3.25 onward to prevent overweight.  The last dog in the control group died at 13.3 years, while the last dog in the CR group lived until it was “nearly 15”… about a year-and-a-half longer, or about 11% longer than the slightly restricted control dog. The median age at death for the CR group was 22 months (15%) greater than the median age for the control group. When only one member of the control group was left, 18 members of the CR group were still running around. When the last member of the control group had died, 6 of the CR Labradors were still living. And the health of CR group was much better than that of the control group at every age.

The maximum life span, defined as the average age at which the longest-lived 10% died, was a little longer in the CR group but the difference wasn’t statistically significant at the p<0.05 confidence level (although the oldest retriever in the CR arm outlived the oldest dog in the control group by about 18 months). Of course, the longest-lived 10% in each group consisted of 2.4 dogs, which doesn’t lend itself to very accurate statistics.

The key issue was that although the CR group outlived the control group, they did so by 10%-to-15%, and not by the 25% that you’d have expected in mice, or about 50% to 60% of the life span extension that you’d expect in mice. These Labrador retrievers lived about 5 times longer than mice, and about 1/6th as long as humans.

It's worth noting that

Otherwise, I consider this study to be an endorsement for CR.

The Okinawans

The Okinawans practice 20% calorie restriction (38% as children), eat at least 7 servings of vegetables and 5 servings of fruit a day, eat fish several times a week (along with very little meat), exercise, and live laid back and sociable lives. They also drink turmeric tea (and presumably, green tea). Not only do they live a long time, they’re also very healthy and active into their nineties.

It might be worth noting that their 20% calorie reduction is measured with respect to the calorie intake of the average Japanese But given the Okinawans' childhood food restraint, they might be shorter and smaller than the Japanese, in which case, their normal adult calorie requirement might be a bit less than that of the typical Japanese. At 20% fewer calories than the Japanese, how calorie-restricted are they? To know the answer to that question, we would have to know their their percentages of body fat. (That information may already be available as a part of the Okinawa Centenarian Study.) It's also important to note that younger Okinawans  have become overweight junk food addicts, so population averages are apt to be skewed.

In 1960, the life expectancy for the average Okinawan was 72 when Japan’s life expectancy was 67.5, Sweden’s life expectancy was 73 and the U. S. stood at not-quite 70.  By 1998, Okinawa had reached 81.2, Sweden was at 79, the U. S. was about 76, and Japan was about 80. The Okinawans had surpassed the Swedes to become the longest-lived people in the world. But this is a case of “damned with faint praise”.  Given their presumed-to-be-extremely-healthy lifestyles (never mind calorie restriction), why aren’t they living longer than 81.2 years? Long-term-vegetarian California Seventh-Day Adventists had an average life expectancy of 84.5 years[9], and, perhaps, higher if they ate nuts and exercised. In fact, "the life expectancies of California Adventist men and women are higher than those of any other well-described natural population" (from speeches at Oxford Vegetarians).  Furthermore, the life expectancies of the Okinawans rose very rapidly since 1960, and this among a people who, supposedly, already practiced some of the best health habits in the world.  So what’s going on?  

And the next question is: what does this life expectancy mean? Is it a projection of future life expectancies, measured from 1998 onward (to the year 2079.2)? If so, how did the actuaries factor in the overweight condition of younger Okinawans? On the other hand, if it's the average lifespan of people who lived until 1998, it would be incorrect to compare it with the future projections of lifespans in other countries.

I’m suspicious about comparisons like this between the third-word agrarian societies and first-world post-industrial societies. The Okinawans may be doing a lot better than casually meets the eye, a third-world player beating the first-world players at their own game. Life expectancies jumped sharply in first-world countries because of clean water supplies, childhood vaccination programs, public health programs, and personal hygiene during the first half of the twentieth century. What was happening in Okinawa during those years? What about bacterially contaminated water? What about parasites such as body lice, and infectious loads?

How much should 20% calorie restriction affect their life spans? First, we would have to know what their life spans would be without calorie restriction. Okinawa’s culture is separate from the rest of Japan so it’s not easy to say.  The U. S. arrived during World War II and stayed until 1978, so penicillin would have been introduced to Okinawa around 1945, but not necessarily to the indigenous population. If calorie restriction worked as it does in mice, it would have extended their life spans by 20%, from 67.7 to 81.2. If it worked the way it does in Labrador retrievers, it would have extended their life spans by 10%, from 73.8 to 81.2. Both 67.7 sad 73.8 would be respectable life expectancies for rural third-world countries.

It’s worth noting that, Kamato Hongo, the world’s oldest person for several years, hailed from an island near Okinawa.  Ms. Hongo drank green tea and herbal (turmeric?) tea. The oldest Okinawan so far lived to 113 out of a population of about 1.25 million inhabitants. Another one was 108 and was going fishing every day. Many of the world’s oldest super-centenarians hail from this area.

The bottom line: I don’t know enough of the gritty details of the Okinawa story to assess it.  

But the fact remains that the Okinawans haven't lived 20% longer than the Japanese.

Life Expectancies

One of the first questions to be answered is: what's the maximum life expectancy for humans? It certainly isn't 122. That's the life expectancy of, perhaps, one in hundreds of millions. Also, it isn't the life expectancy of someone born today. That's a projection into the future. If we apply the same yarf stick that we use for animals, the maximum lifespan for all races in the U. S.  would be the average lifespan of the longest-lived 10% of the U. S. population that is reaching that lifespan today, and that number appears to be about 88. (The average lifespan today would be about 70.) This means that a 40% extension of the human lifespan, presumably achievable only by restricting calorie intake 40% or more from the time of weaning, would lead to a maximum (1-in-20) lifespan of about 125 years, and an average lifespan of about 98 years of age. A more reasonable lifespan extension for someone who began 30% CR at the age of 30 (in 1950), might be 15%-to-20%, which would amount to a lifespan extension of 13 to 17 years, leading to a maximum, 1-in-20 age at death of 101 to 105--well within the envelope of current human lifespans. (A 15% to 20% extension of the average human lifespan would be about 10 to 14 years, leading to an average lifespan, if a large population of today's senior citizens had begun practicing 30% CR from age 30 back in the early fifties, of 80 to 84.) For someone starting CR at the age of 50, like Ralph Cornell, the lifespan extension might be expected to be, perhaps, 12 to 15 years.

These numbers are based upon the general population. What about a population that focuses on good health?

 Seventh-Day Adventists who practice what they preach might be a representative population. Their includes good nutrition, moderate exercise, and eating nuts 5 or more times a week (which, for some reason, adds another year-and-a-half to the lifespan). For those surviving to age 30, their health habits add an extra 7.3 years to men and 4.4 years to women. For vegetarians, the gain is 9.5 and 6.1 years, respectively. For someone who is in their mid-30's today, the average life expectancy is about 74. Without CR, the average life expectancy for someone who is 35-37 years old who practices very good health habits, including eating nuts five or more times a week, might be 80, with a maximum (1-in-20) lifespan of, perhaps, 94.  (I'm adding 10 years to the average lifespan but only 6 years to the maximum lifespan because of range compression at upper age levels.) Adding an assumed 13 to 17 years to that assuming that this individual starts CR today might give a projected average lifespan of 93 to 97, and a maximum (1-in-20)  lifespan of about 107 to 111.

A lifespan of 150 years would represent a 70% increase over today's maximum lifespan of 88, and a 23% increase in life span over the one-in-several-hundred-million lifespan of Jean Calment. For several hundred million people starting CR at 35=37, the one-in-several-hundred-million lifespan corresponding to Jean Calment might be 135 to 140... a bit less than the 150-year lifespan projected for CR because of the impracticality of starting CR in humans at weaning. 

An examination of the Center for Disease Control’s Survivorship tables[10] reveals some very interesting information. Out of 100,000 people born in my parents’ birth years, 1900-1902, only 31 of the 100,000 actually became centenarians.  By the time I was born 30 years later, the projected number of centenarians in 2030-2032  had doubled to 62. Today, 70 years after that, the projected frequency of centenarians for babies born in 2100-2102 is 2,100… almost 70 times the number in my parents’ generation!

The average life expectancy is shown as rising from 47 in 1900 to 77 in 2000—an increase of 30 years. (A part of this disparity in lifespans is a consequence of infant mortality. Among people born in 1900-1902, about 20% died before age 20, compared with 2% for infants born today.)

The rise in life expectancy for the longest-lived 10% of the population goes from about 86 in 1900-1902 to a projected  99 for children born in 2000-2002… an increase of about 13 years. And the life expectancy for a centenarian in 2000-2002 is 1.58 years, compared with a projected life span for centenarians of 2.7 years in 2100-2102… a paltry 1.12-year increase.  It would be tempting to conclude that there is an elastic limit a little above 100 to the natural human life span if it weren’t for outliers like 116-year-old Kamato Hongo.

Dr.  Jay Olshansky has argued that these trends, with average life spans rising by ¼-year per year will slow down in the 21st century, and life expectancies might actually fall because of obesity, antibiotic-resistant diseases, etc.


Call By a Well-Known Life Extension Skeptic for a $3,000,000,000 Federal Program to Slow the Rate of Aging by Seven Years

Dr. Olshansky has recently called for a $3,000,000,000 program to try to slow human aging by 7 years (8%? 9%?).[11]

Monkey Business

In 1987, Drs. George Roth, Donald Ingram and Mark Lane at the National Institute on Aging began a study of long-term calorie restriction with 30 monkeys. This has since expanded to nearly 200 monkeys with three basic age cohorts: very young, young-adult, and old (equivalent to the age of 60 in humans). From the program’s inception, the CR group has exhibited the characteristics common to other species on CR: lowered fasting insulin levels, lowered fasting blood sugar levels, lowered body temperatures, and elevated HDL, and insulin sensitivity, to name a few. Dehydroepiandrosterone (DHEA) and alkaline phosphatase levels in the CR monkeys, thought to be meaningful barometers of aging, aren’t declining with age the way they are in the full-calorie group. The CR monkeys are smaller, younger-looking, and healthier than the ad libitum-fed monkeys. The CR monkeys have about 7% body fat, in comparison with 15%-20% body fat among the controls.

Some accidental deaths have occurred over the years, such as overcooked food causing deadly gastric bloat. This might muddy the waters when the time comes to crunch the final numbers.

These monkeys are now 19 years into the study, with average life spans of about 27 years, and “maximum” life spans (defined as the average age at death of the longest-lived decile) of...  35(?) years (corresponding to human ages of 75 and 97-). It will be 2014 before they reach the 27-year mark.

At the Fourth Calorie Restriction Conference, it was revealed that the control group of monkeys in the NIA study are becoming so depressed in their middle age, cooped up in their cages, that they’re eating no more than the CR monkeys. They have to be cajoled, and fed candy to get them to eat. They throw their food at the walls and at their caretakers and act angry and depressed, as though they're tired to death of being penned up all their lives in cages. Even the calorie-restricted monkeys aren't eating all their food.

In the meantime, at the University of Wisconsin, about 30% of the fully fed monkeys have died, versus 10% of the calorie-restricted monkeys. The CR monkeys have had two cases of cancer versus five in the controls (out of 76 monkeys). The controls have had twice the death rate from aging -related diseases like heart failure and diabetes.

The oldest member of the CR group, C58, set a new longevity record by dying last year at the age of 41. The second-oldest confirmed monkey life span was 36, with an unconfirmed report of another monkey who reached 39. These latter two monkeys were fully fed, but they’re also the oldest monkeys on record (1 in 1,000? 1 in 10,000?) Note that C58 presumably wouldn’t have been calorie restricted until its last 18 years, when the NIA Aging in Non-Human Primates program began. That means it would have been about 23, equivalent to 69 in human terms, when its calorie restriction began.

These non-human primates seem to me to be a litmus test regarding whether or not CR will slow aging in humans. 

National Institute on Aging researchers Don Ingram, George Roth, Matt Lane, M. A. Ottinger, S. Zou, R. de Cabo, and J. A. Mattison have just pre-published a Biogerontology paper 

"Based on results emerging from long-term studies of dietary restriction in rhesus monkeys, we offer our views regarding whether dietary restriction can increase longevity in humans. Because lifespan data in monkeys remain inconclusive currently, we respond that 'we do not for sure.' Based on the vast literature regarding the effects of healthy, low calorie diets on health and longevity in a wide range of species, including humans, and based on data emerging from monkey studies
suggesting that dietary restriction improves markers of disease risk and health, we respond that 'we think so.'"  


In rhesus monkeys, calorie restriction has had pronounced effects. A University of Wisconsin team led by Richard Weindruch has been studying 76 monkeys for more than a decade, half on low-calorie diets and half in a control group that eats normally.

The low-calorie animals weigh about 30 percent less and have 70 percent less body fat than the controls, as well as lower insulin levels. The calorie-restricted monkeys have had two cases of cancer, compared with five in the controls. The controls have had twice the death rate from aging-related diseases like heart failure and diabetes. About 90 percent of the monkeys on low-calorie diets are still alive, compared with only about 70 percent of the controls.

"Evidence emerging from studies in rhesus monkeys suggests that their response to CR parallels that observed in rodents."

Animal research suggests a number

Of course, even if CR doesn’t slow the rate of aging in humans, its health benefits, and its ability to extend your life span would seem to me to be  more than worth the price of admission.

Summing It Up

Whether or not CR slows aging in humans, if it gives us, as individuals, another ten or fifteen years of healthy living, that will be good enough for now (2006). And the effects of CR in humans are dramatic. The Washington University researchers continue to find evidence that calorie restriction in humans produces the same kinds of fundamental changes in humans that they do in smaller mammals.  In my opinion, the idea that CR will have little or no effect in humans won’t hold water, nor will the idea that CR started later in life will afford minimal or no benefits. My own experience and that of others such as Ralph Cornell suggests otherwise.

 For the purpose of allocating research priorities, I think it’s important that we know whether CR slows aging, or whether it merely improves health and enables us, as Bob Cavanaugh puts it, to reach our full genetic potential. 

If CR extends average life span without slowing the rate of aging and extending maximum life span, then we have to postulate two different kinds of calorie restriction effects: one kind that increases average life span and another kind that extends maximum life span. I could certainly be wrong, and I may change my tune without warning as I learn more, but right now, I don’t think that’s the case. I suspect that CR will shift the survival curve to the right, just as it does in all other species that have been tested to date, rather than changing the shape of the curve. I also speculate that it will slow aging in humans and extend maximum life span by something like the same percentage that it increases maximum life span. It may be that maximum human life spans are extended by only 10% or 15% for 30% calorie restriction, rather than the 30% maximum life extensions that are observed in rats and mice, but I think there will be some sizable effect and I think that much of this effect will occur for those who start CR later in life. I’m heartened by the story of C58, above. C58 has already set a new record for rhesus monkey longevity and the program is just getting started. 
    Also, it’s hard to imagine the resounding improvements in so many age-related parameters having no influence upon maximum human life span as well as average life span. 

Based upon my review of the literature for this article, I'm still hopeful that CR may add another 15 to 25 years to our lives, 10 or 15 of which would be the result of the slowing of aging.
__________________________________________________________________________________________

[1]  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VRT-4J6NSR8-12&_coverDate=
[2] - http://www.sciencedaily.com/releases/2006/02/060206232855.htm
[3]  - http://www.pnas.org/cgi/content/abstract/0308291101v1
[4]  - http://www.sciencedaily.com/releases/2006/01/060113112537.htm
[5] - http://www.relentlessimprovement.com/learn/rlipoic_faq.php
[6] - http://www.protandim.com/
[7] - http://www.protandim.com/pdf/Protandim%20FRBM%202006.pdf
[8] - http://search.lef.org/cgi-src-bin/MsmGo.exe?grab_id=0&page_id=1360&query=SOD&hiword=SOD%20
[9] - http://www.davidephraim.com/NEWS/health_news.htm
[10] - http://www.cdc.gov/nchs/data/nvsr/nvsr53/nvsr53_06.pdf
[11] - http://www.fightaging.org/archives/000781.php